07.100.10 医学微生物学 标准查询与下载

GOST R 51758-2001 兽医用营养培养基.生物学试验方法

Настоящий стандарт распространяется на питательные среды для микроорганизмов и культур клеток, а также основные компоненты питательных

Nutrient media for veterinary purposes. Methods of biological testing

LST EN 12322-2000 体外诊断医疗器械 微生物学培养基 培养基性能标准

In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media

ASTM E1533-00 用4'-6-二氨基-2-2苯基吲哚（DAPI）染色间接检测细胞培养中支原体的标准实施规程

1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining. 1.2 This practice does not cover direct methods for the detection of mycoplasma or other indirect methods such as enzymatical detection or DNA probes. 1.3 This practice does not cover methods for the identification of mycoplasma organisms. 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only. 1.5 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4'-6-Diamidino-2-2 Phenylindole (DAPI) Staining

ASTM E1532-00(2006) 用二苯甲酰胺与DNA结合荧光染料法检测细胞培养中支原体污染物的标准实施规程

Mycoplasma hyorhinis, cultivar α strains (1)3 do not grow on any of the standard media used for mycoplasma cultivation. These strains, which are found as contaminants in cell cultures, are detected by indirect methods. A specialized medium has been described but it is not yet in wide use (2). This practice should be used in conjunction with Practice E 1531. All cell cultures to be examined for mycoplasma should undergo a minimum of two passages in antibiototic-free tissue culture medium before testing.1.1 This practice covers the use of cell cultures and DNA-binding flurorochrome techniques to detect mycoplasma contamination of cell cultures.1.2 This practice does not cover axenic cultivation or identification of mycoplasmas.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Practice for Detection of Mycoplasma Contamination of Cell Cultures by Use of Bisbenzamide DNA-Binding Fluorochrome

ASTM E1536-00(2006) 用大容量试验法探测牛血清支原体污染物的标准实施规程

Mycoplasmas of bovine origin are prevalent contaminants of cell cultures. Contamination can be detected by the large volume method.3 ,4 Heat inactivated serum need not be tested for mycoplasmas. Heating serum to 56°C for 30 min will kill mycoplasmas. Mycoplasmas may be present in any particular lot of serum but may not be detected because of inadequate sample size; thus, negative test results do not provide absolute assurance that the test serum is free of mycoplasmas.1.1 This practice covers the procedures used for detection of mycoplasma contamination in serum by direct microbiological culture.1.2 This practice does not cover procedures used for detection of mycoplasma in cell cultures.1.3 This practice does not cover indirect methods for detection of mycoplasma contamination.1.4 This practice does not cover methods for identification of mycoplasma cultures.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Practice for Detection of Mycoplasma Contamination of Bovine Serum by Large Volume Method

ASTM E1533-00(2006) 4-6-二醚基-2-2-苯胺基(DAPI)染色法间接检测细胞培养中菌质的标准实施规程

Mycoplasma contamination of cell cultures is a common problem that can affect the growth, metabolism, and function of cultured animal cells. The ability to detect mycoplasma in cell cultures provides an opportunity to ensure that cells are free of contamination, and to replace those that are not. For additional information, see Practices E 1531, E 1532, and E 1536. Strict adherence to established, well-tested procedures is necessary. This practice was developed by Task Group E48.01.02 to assist in developing and maintaining an established regimen for mycoplasma detection by indirect 4′-6-Diamidino-2-Phenylindole (DAPI) fluorochrome staining. This practice is intended for use in examining cultured animal cells for the presence of mycoplasma contamination. This practice is not intended for use in the detection of mycoplasma contamination in serum, culture media, or systems other than cultures of animal cells. All cell cultures to be examined for mycoplasma should undergo a minimum of two passages in antibiotic-free tissue culture medium before testing.1.1 This practice covers procedures used for the detection of mycoplasma contamination by indirect DNA staining.1.2 This practice does not cover direct methods for the detection of mycoplasma or other indirect methods such as enzymatical detection or DNA probes.1.3 This practice does not cover methods for the identification of mycoplasma organisms.1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Practice for Indirect Detection of Mycoplasma in Cell Culture by 4`-6-Diamidino-2-2 Phenylindole (DAPI) Staining

ASTM E1286-00(2006) 单纯疱疹病毒或它的脱氧核糖核酸的识别用标准指南

This guide is intended for use in a biotechnology laboratory whenever the necessity arises for identifying a biological preparation believed to contain primarily HSV or its DNA. The characterization criteria used for the identification shall be performed by an individual trained in molecular virology. This guide is not meant to be used in a clinical laboratory for the identification of HSV isolated from patient specimens. 1.1 This guide covers laboratory characterization procedures sufficient to identify purified specimens of HSV types 1 and 2 (HSV-1 and HSV-2) or HSV-1 DNA and HSV-2 DNA used in biotechnology. For cases in which identification of HSV DNA specimens is required, the characterization criteria of and of this guide are sufficient.1.2 This guide does not cover the identification of HSV in HSV-infected host cells. To apply this guide to such a case, it would first be necessary to isolate the virus from such samples using standard techniques of HSV purification. This guide does not cover characterization of segments of HSV DNA or of vectors containing HSV DNA segments.1.3 This guide does not cover the specific methodology used in the identification characterization. It does not address the question of degree of purity required for herpes virus preparations: this would vary depending on the particular biotechnology use of the virus.1.4 Warning-Laboratory work involving herpes simplex viruses can be hazardous to personnel. Biosafety 2 level facilities are recommended (). Safety guidelines shall be adhered to according to NCCLS M29-T2 and other recommendations (). This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Standard Guide for Identification of Herpes Simplex Virus or Its DNA

DS/EN 12322:1999 体外诊断医疗器械 微生物学培养基 培养基性能标准

This standard specifies requirements for the performance of culture media. It is concerned with the traceability, comparability, reproducibility and suitability of culture media used in microbiological laboratories.

In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media

BS EN 12322:1999 体外诊断医疗装置.微生物培养基.培养基性能标准

This European Standard specifies requirements for the performance of culture media. It is concerned with the traceability, comparability, reproducibility and suitability of culture media used in microbiological laboratories. These characteristics are achieved by applying the quality criteria outlined in this standard. This European Standard is applicable to: a) commercial organizations distributing media to microbiology laboratories in ready-to-use form, as dehydrated media or as semi-finished media (see 2.5 in EN 1659:1996); b) non-commercial organizations that distribute media to satellite locations; c) laboratories that prepare culture media for their own use. Cell culture media are not covered by this standard.

In vitro diagnostic medical devices. Culture media for microbiology. Performance criteria for culture media

DIN EN 12322:1999 体外诊断医疗装置.微生物用培养基.培养基的功能标准

In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media; German version EN 12322:1999

EN 12322:1999 试管诊断医疗装置.微生物用培养基.培养基的功能标准.包含修改件A1-2001

This European Standard specifies requirements for the performance ofculture media. It is concerned with the traceability, comparability, reproducibility and suitability of culture media used in microbiological laboratories. These characteristics are achieved by applying the quality criteria outlined in this standard.This European Standard is applicable to:a) commercial organizations distributing media to microbiology laboratories in ready-to-use form, as dehydrated media or as semi-finished media (see 2.5 in EN 1659:1996);b) non-commercial organizations that distribute media to satellite locations;c) laboratories that prepare culture media for their own use

In vitro diagnostic medical devices - Culture media for microbiology - Performance criteria for culture media

DIN 58940-10 Bb.1:1998 医用微生物学.病源细菌(分支杆菌除外)对化疗敏感性的测定方法.第10部分:新化疗剂实验室效应的评定标准.补充件1:数据表

Medical microbiology - Susceptibility testing of pathogens to antimicrobial agents - Part 10: Criteria for the evaluation of the in vitro efficacy of new antimicrobial agents; Supplement 1: Data form sheets

DIN 58959-19 Bb.1:1997 医疗微生物学质量管理.第19部分:真菌研究对照菌株要求.常用培养基和试剂对照菌株

Quality management in medical microbiology - Part 19: Requirements for the use of control strains for the examination of fungi; control strains for commonly used culture media and reagents

DIN 58959-2:1997 医疗微生物学质量管理.第2部分:试样采集、运输和验收要求

The document applies to all specimens sent for examination to a medical microbiological laboratories according to DIN 58956-1. It specifies minimum requirements for the quality of biological specimens, specimen sampling, specimen transport and acceptance criteria for specimens as well at the possible resulting rejection of specimens to be examined in the medical microbiological laboratory.

Quality management in medical microbiology - Part 2: Requirements for sample collection, transport and acceptance of specimens

DIN 58959-7 Bb.3:1997 医疗微生物学质量管理.第7部分:使用对照菌株的一般要求.试验结果文件对照卡实例

Quality management in medical microbiology - Part 7: General requirements for the use of control strains; examples for control cards documentation of test results

DIN 58959 Bb.1:1997 医疗微生物学质量管理.综述和索引

Quality management in medical microbiology - Overview and index

DIN 58959-6 Bb.2:1997 医疗微生物学质量管理.第6部分:对照菌株要求.菌株培养和应用培养的真菌的生产和保管实例

Quality management in medical microbiology - Part 6: Requirements for control strains; examples for production and preservation of fungi used as stock cultures and working cultures

DIN 58959-7 Bb.1:1997 医疗微生物学质量管理.第7部分:使用对照菌株的一般要求.对照菌株供应源

Quality management in medical microbiology - Part 7: General requirements for the use of control strains; sources of supply for control strains

DIN 58959-7 Bb.2:1997 医疗微生物学质量管理.第7部分:使用对照菌株的一般要求.常用对照菌株的ATTC编号和DSM编号

Quality management in medical microbiology - Part 7: General requirements for the use of control strains; ATTC and DSM numbers of commonly used control strains

DIN 58959-14 Bb.1:1997 医疗微生物学质量管理.第14部分:需氧菌敏感性检验对照菌株要求.需氧菌用MHK/HHD-极限(控制)区

Quality management in medical microbiology - Part 14: Requirements for the use of control strains for susceptibility testing of aerobic bacteria; MIC/IZD limits for aerobes