T Cell Culture for Gammaretroviral Transfer
关键词: culture gammaretroviral transfer来源: 互联网
Gene transfer into mature T cells with gammaretroviral vectors requires prestimulation, as only mitotic cells are susceptible to integration of the gammaretroviral proviral genome. Costimulation via the CD3/ TCR complex and a second costimulatory molecule, such as CD28 was found to better preserve functionality of the T lymphocytes during ex vivo expansion than stimulation with anti-CD3 alone. The protocols described here for prestimulation and transduction of human and murine T cells with gammaretroviral vectors were optimized for high-level gene transfer and maximum yield of functional T lymphocytes.
推荐方法
- Isolation and Culture of Porcine Embryonic Stem Cells
- Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast
- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay
- Retroviral-Mediated Transduction and Clonal Integration Analysis of Human Hematopoietic Stem and Progenitor Cells
- Single-Cell Culture in Microwells
- The Evolution of Mass Spectrometry in the Clinical Laboratory
- Purification and Depletion of RNP Particles by Antisense Affinity Chromatography
- Analysis of DNA Topology in Yeast Chromatin
- Plasmid Replicon Typing
- Gene Transfer Via Nucleofection Into Adult and Embryonic Stem Cells