Homologous Gene Targeting to Study ECM Assembly
Genetically engineered mutant strains of mice have become invaluable too to decipher complex biological processes, such as ECM assembly, and to dissect phenotypic overlaps, like the genodermatoses. Homologous gene targe ing in embryonic stem (ES) cells is increasingly favored over the transgen approach because it allows the study of mutations in homozygosity and without altering the remainder of the genome (1). Homologous gene targeting has been successfully employed to abrogate the expression or alter the structure of ECM-coding genes (2,3). In both cases, the experimental protocol consists of the following three steps: a. construction of the targeting vector; b. homologous recombination in ES cells; and c. generation of mutant mouse strains.
- Isolation and Maintenance of Mouse Epiblast Stem Cells
- Isolation and Characterization of Mouse Side Population Cells
- Gene Dosage in Capillary Electrophoresis: Prenatal Diagnosis of Downs Syndrome and Rh D/d Genotyping
- Generation of Induced Pluripotent Stem Cells from Human Amnion Cells
- Padlock Probes and Rolling Circle Amplification for Detection of Repeats and Single-Copy Genes in the Single-Cell Comet Assay
- A High Content Imaging-Based Approach for Classifying Cellular Phenotypes
- Heart Xenotransplantation in Primate Models
- Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Using the Budding Yeast Saccharo
- 细胞培养中常用培养基及基本特性
- PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS