Composition per liter:

NaCl ……19.45g

Agar ……15.0g

MgCl2……8.8g

Peptone ……5.0g

Na2SO3……3.24g

CaCl2……1.8g

Yeast extract……1.0g

KCl……0.55g

NaHCO3……0.16g

Ferric citrate……0.1g

KBr ……0.08g

SrCl2……0.03g

H3BO3 ……0.02g

Na2HPO4……8.0mg

Na2SiO3……4.0mg

NaF ……2.4mg

NH4NO3 ……1.6mg

Biphenyl……1.0mg

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except biphenyl, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure –121°C. Pour into sterile Petri dishes or leave in tubes. After agar solidifies, aseptically add a few crystals of biphenyl to each plate.

Use: For the cultivation and maintenance of biphenyl-utilizing marine bacteria, such as Cycloclasticus pugetii.