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基因表达差异显示实验>Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity
Methods for Diagnostic HLA Typing in Disease Association and Drug Hypersensitivity
关键词: methods diagnostic hla来源: 互联网
This chapter describes the application of diagnostic HLA typing for disease association and five methods used for specific HLA genotypes. The methods utilise a combination of polymerase chain reaction (PCR) amplification to detect sequence polymorphism by the presence or absence of amplification, nucleotide sequencing of the PCR product, and hybridisation of the PCR product with labelled probes. The probes are specific for sequence polymorphism associated with the genotype and are attached to either a Micro Bead or a Solid Phase. In addition, the detection of single nucleotide polymorphism(s) which “tag” for the genotype using a real-time PCR is described.
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