Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarr实验方法详情页

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Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarr

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Abstract
Table of Contents
Materials
Figures
Literature Cited

Abstract

Nature possesses an unlimited number and source of biologically relevant natural glycans, many of which are too complicated to synthesize in the laboratory. To capitalize on the naturally occurring plethora of glycans, a method is presented here to fluorescently tag isolated free glycans while maintaining the closed?ring structure. After purification of the labeled glycans, they can be printed on a glass surface to create a natural glycan microarray, suitable for interrogation with potential glycan?binding proteins. The derivatization of these natural glycans has vastly expanded the number of glycans available for functional studies. Curr. Protoc. Chem. Biol. 3:53?63 © 2011 by John Wiley & Sons, Inc.

Keywords: fluorescence; reductive amination; glycan microarray; conjugation

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Introduction
Basic Protocol 1: Preparation of Closed‐Ring Glycan‐AEAB from Free Reducing Glycans
Alternate Protocol 1: Preparation of AEAB Conjugates Using Commercial Chemicals
Commentary
Literature Cited
Figures

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Basic Protocol 1: Preparation of Closed‐Ring Glycan‐AEAB from Free Reducing Glycans Materials

0.05 to 1 mg free reducing glycan, lyophilized (e.g., Sigma‐Aldrich, V‐labs, Carbosynth)
Milli‐Q purified water (Millipore), or equivalent
Ammonium bicarbonate
50% (v/v) acetonitrile, HPLC grade (Fisher Scientific)/10 mM ammonium bicarbonate
10 mM ammonium bicarbonate
Sodium bicarbonate
Saturated sodium bicarbonate solution, ice‐cold
Acryloyl chloride (e.g., Sigma‐Aldrich)
Sodium borohydride (e.g., Sigma‐Aldrich), optional
Acetic acid, ACS grade (Fisher Scientific), optional
50% (v/v) acetonitrile/0.1% (v/v) trifluoroacetic acid (TFA), HPLC grade (Fisher Scientific)
Methanol
Ethanol
Ozone
Nitrogen gas
Methyl sulfide
7:3 (v/v) dimethyl sulfoxide (DMSO), ACS grade (Fisher Scientific)/acetic acid
Sodium cyanoborohydride
2‐(N ‐aminoethyl)‐amino benzamide (AEAB) hydrochloride (Song et al., )
Acetonitrile
1% (v/v) trifluoroacetic acid (TFA), HPLC grade (Fisher Scientific)

1.5‐ml screw‐cap polypropylene centrifuge tubes
55°C and 65°C heating block or water bath
150 mg, 300 mg, and 1 g carbograph solid phase extraction (SPE) columns (Alltech)
Rotary evaporator (e.g., SpeedVac, Thermo Scientific)
Lyophilizer
15‐ml conical polypropylene centrifuge tubes
Porous graphitized carbon (PGC) analytical HPLC column (Thermo Scientific)
High‐performance liquid chromatography (HPLC) system

Alternate Protocol 1: Preparation of AEAB Conjugates Using Commercial Chemicals Materials

7:3 (v/v) dimethyl sulfoxide (DMSO)/acetic acid solution
p ‐nitrophenyl anthranilate, 98% (PNPA, Fisher Scientific)
Sodium cyanoborohydride, 95% (Sigma‐Aldrich)
0.05 to 1 mg free reducing glycan, lyophilized (e.g., Sigma‐Aldrich, V‐labs, Carbosynth)
Acetonitrile
1% (v/v) trifluoroacetic acid (TFA)
Milli‐Q purified water, or equivalent
Ethylenediamine solution: dissolve 100 µl ethylenediamine in 1 ml DMSO
10% (v/v) acetic acid

65°C heating block or water bath
C18 analytical column
High‐performance liquid chromatography (HPLC) system
Rotary evaporator (e.g., SpeedVac, Thermo Scientific)
Lyophilizer
Hypercarb (PGC) HPLC column

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Figure 1. The closed‐ring fluorescent conjugation of reducing glycans.

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Figure 2. The preparation of AEAB conjugates or fluorescent neoglycoproteins by the reaction of PNPA derivatives with ethylenediamine or proteins, respectively.

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Figure 3. Comparison of the binding of four different antibodies to open‐ring and closed‐ring AEAB conjugates on a glycan array. The arrays were printed using a piezo printer (Perkin‐Elmer) with open‐ and closed‐ring AEAB derivatives at 300 µM on NHS‐derivatized slides. Antibodies were applied to the glycan array at the concentrations indicated in the figure, and detected with appropriate fluorescently labeled secondary antibodies (Song et al., ). The x axis represents different glycans on the array by number, and the y axis represents the relative fluorescence units (RFU) detected on the microarray.

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Literature Cited

Literature Cited
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