Yeast Media
YEPD (non-selection)
| - | 1% yeast extract |
| - | 2% peptone |
| - | 1.5% agar (if needed for plates) |
After autoclaving add glucose to 2% by adding 100 ml of 20% solution to each litre media.
SYNTHETIC MEDIA (selective media)
| - | 0.67% yeast nitrogen base without amino acids (Difco) 0919 15 3 |
| - | 1.5% agar (if needed for plates). Also add 1 pellet of NaOH per litre of agar to avoid mushy plates. |
After autoclaving add 100 ml of 20% glucose (or galactose) solution and 100 ml of 10X Dropout solution to each litre media.
10X DROPOUT SOLUTION
| - | L-Isoleucine 300 mg/L |
| - | L-Valine 1500mg/L |
| - | L-Adenine hemisulfate salt 200 mg/L |
| - | L-Arginine HCl 200 mg/L |
| - | L-Lysine HCl 300 mg/L |
| - | L-Methionine 200 mg/L |
| - | L-Phenylalanine 500 mg/L |
| - | L-Threonine 2000mg/L |
| - | L-Tyrosine 300 mg/L |
| - | L-Uracil 200 mg/L |
| - | L-Histidine HCl monohydrate 200 mg/L* Sigma H9511 |
| - | L-Leucine 1000mg/L* Sigma L1512 |
| - | L-Tryptophan 200 mg/L* Sigma T0271 |
* The 10X Dropout solution does not contain Histidine, Leucine and Tryptophan. These components are used to make selective media to select for yeast transformants.
** A better option is to purchase selective dropout amino acid mix. CSM -His,-Leu,-Trp -- BIO 101 (Cat. 10427-100) and add either His, Leu or Trp as needed.
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