M13噬菌体
· M13 Phage (Michael Blaber)
Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phage culture, it will be great beneficial to read this.
· Large Scale M13RF Isolation (Roe Lab)
After the M13RF containing bacterial cells are harvested by centrifugation, the double-stranded molecules are isolated using the cesium chloride method as for large scale plasmid isolation...
· Phage Miniprep
An improved method for small scale prep. of bacteriophage DNA based on phage precipitation by zinc chloride.
· Single-Stranded M13 DNA Isolation Using Phenol (Roe Lab)
This isolation procedure is the method of choice for preparation of M13-based templates to be used in dye-terminator sequencing reactions.
· Preparation of Single Stranded M13 Template DNA (ScienceXchange)
· PCR of M13 inserts: (GSC, WU)
· Preparation of M13 sublibraries: (GSC, WU)
· Double Stranded DNA Isolation using Phenol(Whitehead Institute/MIT)
· Phagemid Purifications using Streptavidin-coated Magentic Particles(Whitehead Institute/MIT)
· M13 Purifications using Streptavidin Coated Magnetic Particles (Whitehead Institute/MIT)
· M13 RF prep large-scale: (GSC, WU)
· M13 ThermoMAX prep: (GSC, WU)
· M13 helperphage: (GSC, WU)
· M13 PEG prepI: (GSC, WU)
· M13 PEG prepII: (GSC, WU)
· Large-scale M13 prep: (GSC, WU)
· Single Stranded Template from pBluescript Phagemid (NMFS/NOAA)
· Phage Culture and DNA Isolation (ScienceXchange)
· Figure-8 Test (Jim Brown)
The figure-8 test is a test that identifies M13 clones that contain complementary sequences in their viral ssDNA. This occurs when the two clones contain the same region of DNA inserted in opposite orientations. This can be used to identify clones containing the same DNA fragment inserted in opposite directions, to identify clones containing nonidentical but overlapping complementary regions, or to find clones containing portions of DNA also found (complementary) in a larger clone.
· Single Stranded DNA Preparation (Crawford Lab)
· Preparation of ss Phagemid DNA(mini and maxi prep) (Life Technologies)
The protocols can be used to prepare ssDNA from DH5aF', DH5aF'IQ, DH11S, or DH12S cells.
· Phage Purification with DE52 (PMCI Research)
· Colony/Plaque Blotting (Gimila's Lab)
