德国IBL ASP ELISA试剂盒使用说明（一）
ASP (Domoic acid)
direct ELISA
Enzyme immunoassay for the quantitative determination of amnesic
shellfish poison (Domoic acid) in algal, shellfish or seawater extracts.
BS31300401
96
For illustrative purposes only. To perform the assay the instructions for use provided with the kit have to be used. Distributed by: I B L I N T E R N A T I O N A L G M B H Flughafenstrasse 52a Phone: +49 (0)40-53 28 91-0 IBL@IBL-International.com D-22335 Hamburg, Germany Fax: +49 (0)40-53 28 91-11 www.IBL-International.com A S P E L I S A K I T FOR QUANTITATIVE DETERMINATION OF DOMOIC ACID IN SHELLFISH PROD. NO.: A31300401 P R E - R E L E A S E A . I N T R O D U C T I O N Domoic acid (DA) and DA derivatives are water-soluble neurotoxins produced by a number of marine algae, in particular by the microalgae of the genus Pseudonitzschia spp (Fig. 1). Blooms of Pseudo-nitzschia may lead to the accumulation of DA in shellfish filter feeders and other marine species [Scholin et al., 2000]. Ingestion of DA contaminated shellfish may lead to amnesic shellfish poisoning (ASP) by affecting the central nervous system, and has caused the death of both animal and human consumers in severe cases [Wright et al., 1989]. The European commision Directive 2002/226/EC implemented a maximum permitted level (MPL) of 20 mg DA equivalents/kg shellfish intended for human consumption. This MPL is adopted by most food safety authorities.
F I G U R E 1 : D O M O I C A C I D S T R U C T U R E
Enzyme Linked Immunosorbent Assay (ELISA) has proved to be a sensitive and rapid method for detection of DA in the marine environment [Garthwaite et al., 2001]. This quantitative DA ELISA was developed by AgResearch (Hamilton, New Zealand) for the detection of DA in water samples, shellfish and algal extracts, and is based on antibodies described by Garthwaite et al., 1998. The assay is specific for DA, with no cross-reactivity to non-toxic, structural analogues like kainic acid, L-glutamic acid, L-glutamine, formimino-L-glutamic acid, proline or g-aminobutyric acid (GABA). The assay is primarily intended for use in routine monitoring of DA levels in bivalve molluscs to comply with the regulatory MPL, but is also applicable for DA quantification in other marine matrixes. The ASP ELISA has been subjected to comprehensive single-laboratory and inter-laboratory validation studies (Biosense report 2003:6 and 2004:1.2).
