PUBL 33-31826-1986

EVALUATION OF THE POTENTIAL OF PS-8-76D5-ARO TO INDUCE UNSCHEDULED DNA SYNTHESIS IN THE IN VIVO-IN-VITRO HEPATOCYTE DNA REPAIR ASSAY

PUBL 33-31826-1986 发布历史

INTRODUCTION The measurement of chemically induced DNA repair as unscheduled DNA synthesis (UDS) has been shown to be an excellent predictor of genotoxic and carcinogenic potential. A number of in vitro assays for measurement of UDS have been described that employ a variety of cell lines (Trosko and Yager@ 1974; San and Stich@ 1975; Hartin et al.@ 1978)@ including primary cultures of rat hepatocytes (Williams@ 1977). This latter assay offers the advantage of using a target cell that is metabolically competent. Still@ these systems often do not reflect the true genotoxicity present in the whole animal@ and even the in vitro hepatocyte DNA repair assay has been shown to be unresponsive to some classes of compounds@ such as nitroaromatics (Bermudez et al.@ 1979; Probst et al.@ 1981). The in vivo-in vitro hepatocyte DNA repair assay (Mirsalis and Butterworth@ 1980) involves treatment of animals rather than cells in culture and has been shown to be responsive to a wide variety of genotoxic agents@ including nitroaromatics (Mirsalis et al.@ 1982a). In an in vivo system@ factors related to uptake@ metabolic activation@ distribution@ detoxification@ and elimination are inherently accounted for. This assay has been demonstrated to be useful in the study of sex differences@ effects of chronic exposure (Mirsalis and Butterworth@ 1982)@ and the role of activation of compounds by intestinal microflora (Kirsalis et al.@ 1982a). In particular@ this assay is extremely useful for the detection and study of genotoxlc hepatocarcinogens (Mirsalis et al.@ 1982b).

PUBL 33-31826-1986由API - American Petroleum Institute 发布于 1986-06-01，并于 2010-08-10 实施。

PUBL 33-31826-1986的历代版本如下：

• 1986年06月01日 PUBL 33-31826-1986

非常抱歉，我们暂时无法提供预览，您可以试试： 免费下载 PUBL 33-31826-1986 前三页，或者稍后再访问。

注意： 点击下载后，生成下载文件时间比较长，请耐心等待......

标准号

PUBL 33-31826-1986

发布日期

1986年06月01日

实施日期

2010年08月10日

废止日期

无

中国标准分类号

/

国际标准分类号

/

发布单位

API - American Petroleum Institute

引用标准

33

适用范围

INTRODUCTION The measurement of chemically induced DNA repair as unscheduled DNA synthesis (UDS) has been shown to be an excellent predictor of genotoxic and carcinogenic potential. A number of in vitro assays for measurement of UDS have been described that employ a variety of cell lines (Trosko and Yager@ 1974; San and Stich@ 1975; Hartin et al.@ 1978)@ including primary cultures of rat hepatocytes (Williams@ 1977). This latter assay offers the advantage of using a target cell that is metabolically competent. Still@ these systems often do not reflect the true genotoxicity present in the whole animal@ and even the in vitro hepatocyte DNA repair assay has been shown to be unresponsive to some classes of compounds@ such as nitroaromatics (Bermudez et al.@ 1979; Probst et al.@ 1981). The in vivo-in vitro hepatocyte DNA repair assay (Mirsalis and Butterworth@ 1980) involves treatment of animals rather than cells in culture and has been shown to be responsive to a wide variety of genotoxic agents@ including nitroaromatics (Mirsalis et al.@ 1982a). In an in vivo system@ factors related to uptake@ metabolic activation@ distribution@ detoxification@ and elimination are inherently accounted for. This assay has been demonstrated to be useful in the study of sex differences@ effects of chronic exposure (Mirsalis and Butterworth@ 1982)@ and the role of activation of compounds by intestinal microflora (Kirsalis et al.@ 1982a). In particular@ this assay is extremely useful for the detection and study of genotoxlc hepatocarcinogens (Mirsalis et al.@ 1982b).