Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after precolumn derivatization
This Technical Specification specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2
(FB2) in processed maize-containing foods for infants and young children by high performance liquid
chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection (FLD). This method has
been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples
ranging from 112 μg/kg to 458 μg/kg for FB1+FB2, 89 μg/kg to 384 μg/kg for FB1 and 22 μg/kg to 74 μg/kg for
For further information on the validation see Clause 8 and Annex B.