This standard specifies the HPLC method for the determination of thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine in health food. This standard is applicable to the HPLC determination method of thiamine hydrochloride, pyridoxine hydrochloride, niacin, niacinamide and caffeine as dietary supplements added to tablets, capsules, oral liquids, beverages and other sample types. The detection limit of this method is: 0.02 μg of thiamine hydrochloride, 0.02 μg of pyridoxine hydrochloride, 0.10 μg of nicotinic acid, 0.06 μg of nicotinamide, and 0.05 μg of caffeine. Linear range: thiamine hydrochloride 0.500μg/Ml~50.0μg/Ml; pyridoxine hydrochloride 0.500μg/Ml~50.0μg/Ml; niacin 1.00μg/Ml~100μg/Ml; nicotinamide 1.00μg/Ml~100μg /Ml; caffeine 5.00 μg/mL~500 μg/mL.
GB/T 5009.197-2003 history
2003GB/T 5009.197-2003 Determination of thiamine hydrochloride,pyridoxine hydrochloride,niacin niacinamide and caffeine in health foods