07.100.20 水的微生物学 标准查询与下载

T/ZAQ 10117-2023 水体中微囊藻基因的测定 实时荧光定量PCR法

本标准规定了测定水中总微囊藻基因和产毒微囊藻基因测定的方法原理、试剂和材料、仪器与设备、样品、分析步骤、精密度和准确度、质量保证与控制等。

Determination of Microcystis Gene in Water Real-time Quantitative PCR methods

T/ZZB Q066-2023 水中微囊藻基因的测定 实时荧光定量PCR法

本标准规定了测定水中总微囊藻基因和产毒微囊藻基因测定的方法原理、试剂和材料、仪器与设备、样品、分析步骤、精密度和准确度、质量保证与控制等。 本标准适用于水中总微囊藻基因和产毒微囊藻基因的测定。

Determination of Microcystis Gene in Water Real-time Quantitative PCR methods

ISO/CD 13647 水质 可培养微生物的计数 通过在 R2A 培养基上接种扩散板进行菌落计数

Water quality — Enumeration of culturable microorganisms — Colony count by spread plate inoculation on R2A medium

ISO/CD 9308-2 水质《大肠杆菌和大肠菌群计数》第2部分：最可能数法

Water quality — Enumeration of Escherichia coli and coliform bacteria — Part 2: Most probable number method

ISO/AWI 11133 食品、动物饲料和水的微生物学 培养基的制备、生产、贮存和性能检测

Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media

ISO/CD 7899-3 水质《肠道肠球菌计数》第3部分：最可能数法

Water quality — Enumeration of intestinal enterococci — Part 3: Most probable number method

BS EN ISO 7704:2023 水质 培养法直接计数微生物用膜过滤器性能测试要求

Water quality. Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods

EN ISO 7704:2023 水质 用于微生物学分析的滤膜过滤器的评价方法

This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods. This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19]. This document is not applicable for membrane filters used for concentration and elution or for qualitative methods. These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as: — drinking water, bottled water and other types of water with expected low numbers of microorganisms; — water with expected higher numbers of microorganisms, for example, surface water and process water. These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13]. This document applies to: — manufacturers producing membrane filters; — microbiological laboratories using membrane filters for their own testing or providing these to other end users.

Water quality - Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods (ISO 7704:2023)

ISO 7704:2023 水质 用于微生物学分析的滤膜过滤器的评价方法

Water quality — Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods

ASTM E1023-23 评估材料对水生生物及其用途的危害的标准指南

1.1 This guide describes a stepwise process for using information concerning the biological, chemical, physical, and toxicological properties of a material to identify adverse effects likely to occur to aquatic organisms and their uses as a result of release of the material to the environment. The material will usually be a specific chemical, although it might be a group of chemicals that have very similar biological, chemical, physical, and toxicological properties and are usually produced, used, and discarded together. 1.2 The hazard assessment process is complex and requires decisions at a number of points; thus, the validity of a hazard assessment depends on the soundness of those decisions, as well as the accuracy of the information used. All decisions should be based on reasonable worst-case analyses so that an appropriate assessment can be completed for the least cost that is consistent with scientific validity. 1.3 This guide assumes that the reader is knowledgeable in aquatic toxicology and related pertinent areas. A list of general references is provided (1).2 1.4 This guide does not describe or reference detailed procedures for estimating or measuring environmental concentrations, or procedures for determining the maximum concentration of test material that is acceptable in the food of predators of aquatic life. However, this guide does describe how such information should be used when assessing the hazard of a material to aquatic organisms and their uses. 1.5 Because assessment of hazard to aquatic organisms and their uses is a relatively new activity within aquatic toxicology, most of the guidance provided herein is qualitative rather than quantitative. When possible, confidence limits should be calculated and taken into account. 1.6 This guide provides guidance for assessing hazard but does not provide guidance on how to take into account social considerations in order to judge the acceptability of the hazard. Judgments concerning acceptability are social as well as scientific, and are outside the scope of this guide. 1.7 This guide is arranged as follows: Section Referenced Documents 2 Descriptions of Terms Specific to This Standard 3 Summary of Guide 4 Significance and Use 5 Four Basic Concepts 6 The Iteration 6.1 The Two Elements 6.2 The Possible Decisions 6.3 The Phased Approach 6.4 Phase I—Use of Low-Cost (Existing) Information 7 Collection of Available Data 7.1 Initial Estimates of Environmental Concentrations 7.2 Initial Estimate of Toxicity to Aquatic Organisms 7.3 Initial Estimate of Bioaccumulation by Aquatic Organisms 7.4 Phase I Hazard Assessment 7.5 Phase II—Use of Medium-Cost Information 8 Improved Estimates of Environmental Concentrations 8.2 Acute Toxicity to Aquatic Animals 8.3 Toxicity to Algae 8.4 Expansion of Short-Term Testing 8.5 Bioaccumulation 8.6 Phase II Hazard Assessment 8.7 Phase III—Use of High-Cost Information 9 Refined Estimates of Environmental Concentrations 9.2 Chronic Toxicity to Aquatic Animals 9.3 Use of Acute-Chronic Ratios 9.4 Toxicity to Aquatic Plants 9.5 Bioconcentration 9.6 Bioaccumulation from Food 9.7 Phase III Hazard Assessment 9.8 Appendixes Appendix X1 Production, Use, Disposal, and Other Release Appendix X2 Biological Considerations Appendix X3 Chemical Considerations Appendix X4 Physical Considerations Appendix X5 Toxicological Considerations Appendix X6 Estimating Environmental Concentrations Appendix X7 Selection of Test Species Appendix X8 Long-Term Toxicity Tests 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2023. Published February 2023. Originally approved in 1984. Last previous edition approved in 2014 as E1023-84(2014). DOI: 10.1520/E1023-23. 2 Boldface numbers in parentheses refer to the list of references at the end of this standard. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses

GSO ISO 12869:2022 水质 军团菌的检测和定量 和/或嗜肺军团菌 通过定量聚合酶链反应 (qPCR) 进行浓缩和基因扩增

This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements. Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable. NOTE 1 For performance requirements, see Clause 9. This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit. NOTE 2 For validation requirements, see 9.7. The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample. The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method. The qPCR methods do not give any information about the physiological state of the Legionella.

Water quality — Detection and quantification of Legionella spp. and/or Legionella pneumophila by concentration and genic amplification by quantitative polymerase chain reaction (qPCR)

GSO ISO 11731:2022 水质 军团菌计数

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples. These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc. Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

Water quality — Enumeration of Legionella

GSO ASTM F838:2022 测定液体过滤用膜过滤器的细菌截留量的标准试验方法

  Scope is not provided for this standard

Standard Test Method for Determining Bacterial Retention of Membrane Filters Utilized for Liquid Filtration

ASTM E2783-22 用杀时程序评定水溶性化合物抗菌活性的标准试验方法

1.1 This test method measures the changes of a population of aerobic and anaerobic microorganisms within a specific sampling time when tested against antimicrobial test materials in vitro. The organisms used are standardized as to growth requirements and inoculum preparation and must grow under the conditions of the test. The primary purpose of this test method is to provide a set of standardized conditions and test organisms to facilitate comparative assessments of antimicrobial materials miscible in aqueous systems. 1.2 This test method allows the option of using a test sample size of 10 mL or 100 mL. 1.3 Knowledge of microbiological techniques is required for this procedure. 1.4 Aseptic technique should be practiced at all times. 1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and SI units follow in parentheses. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Test Method for Assessment of Antimicrobial Activity for Water Miscible Compounds Using a Time-Kill Procedure

BS 7592:2022 BS 7592 军团菌采样水系统中的细菌 实践守则

Find out more about the latest standards  BS 8580-2:2022  and  BS 7592:2022  by checking out our Webinar replay:   Reducing the Risk of Waterborne Infections with British Standards   Download the free brochure to learn more about all the water quality standards. Click   here . What is BS 7592 - Sampling for Legionella bacteria in water systems about? As the number of reported incidences of Legionnaires’ disease continues to rise, BS 7592 gives recommendations and advice on how to sample and test water for the presence of legionella. Who is BS 7592 - Sampling for Legionella bacteria in water systems for? BS 7592 is a key standard for all those involved in water sampling for legionellae, including those taking samples on site and their employers. BS 7592 on sampling for Legionella bacteria in water systems will be used anywhere there is a building water system, and by anyone involved in water sampling for legionellae, including those collecting samples on site and those developing water sampling plans and/or directing where samples are taken from, irrespective of the method of analysis adopted. Users will typically be water supply managers, building engineers and designers, installers and commissioners, risk assessors, Health & Safety inspectors, Environmental Health Officers, health industry managers, building services engineers, water treatment consultants, public buildings owners and operators, anybody ...

BS 7592. Sampling for <i>Legionella</i> bacteria in water systems. Code of practice

ASTM E1295-22 用杜氏石首鱼进行三窝更新毒性试验的标准指南

1.1 This guide describes procedures for obtaining data concerning the adverse effects of an effluent or a test material (added to dilution water, but not to food) on Ceriodaphnia dubia Richard 1894, during continuous exposure throughout a portion of the organism’s life. These procedures should also be useful for conducting life cycle toxicity tests with other Cladocera (Guide E1193), although modifications will be necessary. 1.2 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures, that can be measured accurately at the necessary concentrations in water. With appropriate modifications these procedures can be used to conduct tests on temperature, dissolved oxygen, pH, dissolved ions, and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments (see also Guide E1706), and surface waters. Renewal tests might not be applicable to materials that have high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed, or sorb to test chambers. If the concentration of dissolved oxygen falls below 4 mg/L or the concentration of test material decreases by more than 20 % in test solution(s) at any concentration between renewals, more frequent renewals might be necessary. 1.3 Other modifications of these procedures might be justified by special needs or circumstances. Results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information on new concepts and procedures for conducting three-brood toxicity tests with C. dubia. 1.4 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Reagents and Materials 7 Hazards 8 Dilution Water 9 Requirements 9.1 Source 9.2 Treatment 9.3 Characterization 9.4 Test Material 10 General 10.1 Stock Solution 10.2 Effluent 10.3 Test Concentration(s) 10.4 Collection 10.5 Sample Containers 10.6 Preservation 10.7 Treatment 10.8 Test Organisms 11 Species 11.1 Age 11.2 Source 11.3 Brood Stock 11.4 Food 11.5 Handling 11.6 Quality 11.7 Procedure 12 Demonstration of Feasibility 12.1 Experimental Design 12.2 Dissolved Oxygen 12.3 Temperature 12.4 Preparing Test Solutions 12.5 Conditioning Test Chambers 12.6 Beginning a Test 12.7 Renewing Test Solutions 12.8 Duration of Test 12.9 Biological Data 12.10 Other Measurements 12.11 Test Material 12.12 Analytical Methodology 13 Acceptability of Test 14 Calculation 15 Report 16 Appendixes Food Appendix X1 Culture Techniques Appendix X2 Test Chambers Appendix X3 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Actionand is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2022. Published April 2022. Originally approved in 1989. Last previous edition approved in 2013 as E1295 – 01(2013). DOI: 10.1520/E1295-22. *A Summary of Changes section appears at the end of this standard Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Statistical Guidance Appendix X4 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia

ASTM E1562-22 用多毛环节动物进行急性、慢性和生命周期水生毒性试验的标准指南

1.1 This guide covers procedures for obtaining data concerning the adverse effects of a test material added to marine and estuarine waters on certain species of polychaetes during shortor long-term continuous exposure. The polychaete species used in these tests are either field collected or from laboratory cultures and exposed to varying concentrations of a toxicant in static or static-renewal conditions. These procedures may be useful for conducting toxicity tests with other species of polychaetes, although modifications might be necessary. 1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute, chronic, or life-cycle tests with other species of polychaetes. 1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications, these procedures can be used to conduct these tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, sediments/soils, oil drilling fluids, produced water from oil well production, and other types of industrial wastes. An LC50 (medial lethal concentration) may be calculated from the data generated in each acute and chronic toxicity test when multiple concentrations are tested. Growth, determined by a change in measured weight, and reproduction, as the change in total number of organisms, are used to measure the effect of a toxicant on life-cycle tests; data are analyzed statistically to indicate that concentration at which a significant difference occurs between the test solutions and control(s). 1.4 The results of dose-response acute or chronic toxicity tests with toxicants added experimentally to salt water should usually be reported in terms of an LC50 (mortality), or EC50 (medial effect concentration). The results of life-cycle toxicity tests with toxicants added experimentally to salt water should be reported as that concentration at which a statistically significant difference in the number of offspring or growth (determined by weight) is produced with reference to the control(s). 1.5 Where appropriate, this standard has been designed to be consistent with or complementary to other methods for assessing toxicity to invertebrates described in Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688. 1.6 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Apparatus 6 Facilities 6.1 Construction Materials 6.2 Test Chambers 6.3 Cleaning 6.4 Acceptability 6.5 Safety Precautions 7 Dilution Water 8 Requirements 8.1 Source 8.2 Treatment 8.3 Characterization 8.4 Test Material 9 General 9.1 Stock Solution 9.2 Test Concentrations 9.3 Test Organisms 10 Species 10.1 Age 10.2 Source 10.3 Feeding 10.4 Holding 10.5 Quality 10.6 Procedure 11 Experimental Design 11.1 Acute Test 11.1.1 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Actionand is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2022. Published February 2022. Originally approved in 1994. Last previous edition approved in 2013 as E1562– 00(2013). DOI: 10.1520/E1562-22. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Chronic Test 11.1.2 Life-Cycle Test 11.1.3 Test Condition Specifications 11.2 Dissolved Oxygen 11.2.1 Temperature 11.2.2 Loading 11.2.3 Salinity 11.2.4 Light 11.2.5 Beginning the Test 11.3 Feeding 11.4 Duration of Test 11.5 Biological Data 11.6 Other Measurements 11.7 Hazards Analytical Methodology 13 Acceptability of Test 14 Calculation of Results 15 Report 16 Keywords 17 Appendixes: Neanthes arenaceodentata Appendix X1 Capitella capitata Appendix X2 Ophryotrocha diadema Appendix X3 Dinophilus gyrociliatus Appendix X4 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids

KS I ISO 6461-2021 水质 亚硫酸盐还原厌氧菌（梭菌）孢子的检测和计数

Water quality — Detection and enumeration of the spores of sulfite-reducing anaerobes(clostridia)

KS I ISO 9308-2021 水质 大肠杆菌及大肠菌群的检测与计数

Water quality — Detection and enumeration of Escherichia coli and coliform bacteria

KS I ISO 7899-2021 水质 肠球菌、粪链球菌检测及计数

Water quality — Detection and enumeration of intestinal enterococci and faecal streptococci