400-6699-117转1000
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工商注册信息已核实!参考报价: | 面议 | 型号: | 肿瘤耐药基因检测(KRAS/NRAS/BRAF/EGFR/C-kit/PDGFRA) |
品牌: | Asuragen | 产地: | 美国 |
关注度: | 61 | 信息完整度: | |
样本: | 典型用户: | 暂无 |
400-6699-117转1000
KRAS and BRAF are oncogenes involved in the epidermal growth factor receptor (EGFR) signaling pathway that controls cell proliferation, differentiation and apoptosis. Mutations in the KRAS and BRAF oncogenes are frequently found in human cancers, such as colorectal cancers and non-small cell lung cancers. The Signature® KRAS/BRAF Mutations assay is a research tool for the rapid detection of mutations in genomic DNA extracted from human cell lines or fresh, frozen or fixed tissues.
The qualitative assay simultaneously detects:
6 KRAS mutations in codons 12
6 KRAS mutations in codon 13
1 BRAF mutation in codon 600
The Signature® reagents are adapted for use on the Luminex® 100 IS™ or 200™ Systems.
Assay Features:
Streamlined workflow, protocol and reagents optimized for the molecular lab
Compatible with a broad range of genomic DNA input and type*
FFPE, biopsies, FNA, macro- or micro-dissected cells
6 KRAS mutations in codon 13
Recommended input for optimal performance: 5-20 ng genomic DNA
Qualitative assay with simple data interpretation:
A specimen is positive for a given mutation if signal ≥ validated cut off
Recommended research use cutoff: 400-500 MFI
Analytical sensitivity* of about 1% demonstrated with genomic DNA and model FFPE cell line dilutions
Includes internal endogenous control (EC) to assess sample DNA quality/purity
External positive and negative plate controls provided independently to assess the validity of the amplification, hybridization and detection steps in each run
Assay Controls
The control set provides negative and positive controls for both panels:
G12V Positive Control
G13V Positive Control
BRAF V600E Positive Control
KRAS/BRAF Pooled Control (all mutations detected)
Assay Sensitivity
Analytical sensitivity* of at least 1% with KRAS mutant positive cell line DNA diluted in KRAS WT cell line DNA
Assay Specificity
Excellent analytical specificity* with no cross detection between KRAS and BRAF
The HCT1116 cell line is KRAS G13D positive and wild type BRAF
The HT29 cell line is KRAS wild type and BRAF V600E positive
温馨提示:不可用于临床治疗。
肿瘤耐药基因检测(KRAS/NRAS/BRAF/EGFR/C-kit/PDGFRA)信息由北京阅微基因技术有限公司为您提供,如您想了解更多关于肿瘤耐药基因检测(KRAS/NRAS/BRAF/EGFR/C-kit/PDGFRA)报价、型号、参数等信息,欢迎来电或留言咨询。
注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途