限制性内切酶添加保护碱基数目及酶切效率
限制性核酸内切酶产品选择专题
| &NBS p; 限制性内切酶 保护碱基A-B &NBS p;酶切效率:&NBS p;- 0%&NBS p;- 0-20%- 20-50%- 50-100%*- 反应时间为16小时 酶保护碱基数目12345AarI20-5050-100AasI50-100AatII00-2020-5050-100Acc65I0-2050-100AdeI50-100AluI020-5050-100Alw21I50-100Alw26I50-100Alw44I020-5050-100ApaI50-100BamHI50-100BcnI20-5050-100BclI050-100BcuI50-100BfiI50-100BfmI50-100BfuI50-100BglI20-5050-100BglII050-100Bme1390I20-5050-100BoxI050-100BpiI50-100Bpu10I20-5050-100Bpu1102I50-100BseDI050-100BseGI50-100BseJI050-100BseLI050-100BseMI0-2050-100BseMII50-100BseNI050-100BseSI50-100BseXI20-5050-100Bsh1236I50-100Bsh1285I0-2050-100BshNI50-100BshTI20-5050-100Bsp68I050-100Bsp119I50-100Bsp120I20-5050-100Bsp143I50-100Bsp143II050-100Bsp1407I20-5050-100BspLI50-100BspPI050-100BspTI00-2050-100Bst1107I0-2050-100BstXI050-100Bsu15I50-100BsuRI0-2020-5050-100 |
限制性内切酶保护碱基C-E |
酶切效率 :- 0%- 0-20%- 20-50%- 50-100%*- 反应时间为16小时 酶保护碱基数目12345CaiI00-2050-100CfrI050-100Cfr9I20-5050-100Cfr10I20-5050-100Cfr13I50-100Cfr42I50-100CpoI50-100Csp6I50-100DpnI50-100DraI00-2050-100Eam1104I050-100Eam1105I050-100Ecl136II50-100Eco24I50-100Eco31I20-5050-100Eco32I20-5050-100Eco47I50-100Eco47III00-2050-100Eco52I0-2050-100Eco57I50-100Eco57MI50-100Eco72I0-2050-100Eco81I50-100Eco88I50-100Eco91I20-5050-100Eco105I20-5050-100Eco130I050-100Eco147I050-100EcoO109I50-100EcoRI50-100EherI20-5050-100Esp3rI50-100 |
限制性内切酶保护碱基F-N |
酶切效率 :- 0%- 0-20%- 20-50%- 50-100%*- 反应时间为16小时 酶保护碱基数目12345FaqI0-2050-100FspAI0-2020-5050-100FspBI20-5050-100GsuI50-100Hin1I50-100Hin1II00-2050-100Hin6I00-2050-100HincII50-100HindIII00-2050-100HinfI50-100HpaII0-2050-100HphI050-100Hpy8I0-2050-100HpyF10VI50-100KpnI50-100Kpn2I050-100KspAI20-5050-100LweI20-5050-100MbiI00-2050-100MboI50-100MboII50-100MlsI0-2050-100MluI20-5050-100MnlI050-100Mph1103I20-5050-100MspI0-2050-100MssI20-5050-100MunI20-5050-100MvaI020-5050-100Mva1269I050-100NcoI050-100NdeI *0-2020-5050-100NheI020-5050-100NmuCI20-5050-100NotI20-5050-100NsbI00-2050-100 |
限制性内切酶保护碱基0-X |
酶切效率:- 0%- 0-20%- 20-50%- 50-100%*- 反应时间为16小时 酶保护碱基数目12345OliI0-2020-5050-100PaeI00-2020-5050-100PagI20-5050-100PauI050-100PdiI0-2050-100PdmI50-100PfeI50-100Pfl23II0-2020-5050-100PfoI020-5050-100Psp5II050-100Psp1406I0-2050-100PsuI0-2050-100PstI0-2050-100PsyI050-100PvuI20-5050-100PvuII50-100RsaI50-100SacI50-100SalI20-5050-100SatI050-100ScaI0-2050-100SchI50-100SdaI0-2050-100SduI50-100SfiI50-100SmaI50-100SmiI0-2050-100SmuI50-100SsiI50-100SspI0-2050-100TaaI20-5050-100TaiI50-100TaqI020-5050-100TasI020-5050-100TatI0-2020-5050-100TauI20-5050-100Tru1I00-2050-100Van91I050-100VspI50-100XagI20-5050-100XapI20-5050-100XbaI20-5050-100XceI050-100XhoI0-2050-100XmaJI50-100XmiI50-100I-SceI50-100 |
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