Mouse Renin ELISA Kit英文使用说明
Mouse Renin ELISA Kit使用原理
小鼠肾素(Renin)ELISA试剂盒
货号:FK-QZ3075
规格:96T/48T
Mouse Renin ELISA Kit使用原理
The
kit was a solid phase sandwichenzyme-linked immunosorbent assay
(ELISA), and the AVP concentrations ofthe standard samples and unknown
concentrations of samples were detectedby the addition of microporous
enzyme plates.. At the same time, the AVPand the biotin labeled
antibodies were incubated at the same time..After washing, the addition
of Pro labeled HRP. After the warming andwashing, the removal of the non
binding enzyme conjugate, and then addedto the substrate a, B, and the
enzyme conjugate at the same time.Produce color. The color depth and the
concentration of AVP in thesample was proportional to the concentration
of the sample..
Operation notes
1
reagent should be stored in the label manual, before using to
roomtemperature. The standard after dilute dilute should be discarded,
donot save.
2 the experimental plate should be immediately put back in the bag,seal the preservation, lest metamorphism.
3 no other reagents should be packaged or well. Do not mix differentbatches of reagents. Use before the shelf.
4
use a disposable suction head lest cross contamination, draw
thetermination liquid and substrate B, a liquid, to avoid using the
metalpart of the sample.
5 use clean plastic containers to configure the washing liquid. Allsamples of ingredients and mix well before use in the kit.
6
wash the enzyme plate should be fully dry, do not absorb water
paperdirectly into the enzyme labeled pores in the water absorption.
7
substrate a should be volatile, to avoid prolonged open lid.Substrate B
is sensitive to light, avoiding prolonged exposure to light.To avoid
contact with hand, toxic. After the experiment, read the ODvalue
immediately..
8 add reagents should be consistent in order, to ensure that allreaction plate hole incubation time.
9
to carry out the temperature fertility operation according to thetime,
the amount and the order of the liquid added in the instruction manual.
Kit performance:
1 the correlation coefficient r value of linear regression and theexpected concentration of sample was 0.92 above.
The 2 batch and the batch should be less than 9% and 15% respectively.
Detection range:
1.5 -90 g/ml g/ml
Save condition and its validity:
1 reagent kit preservation: 2-8.
2 validity: 6 months
小鼠肾素(Renin)ELISA试剂盒