EN 12823-1-2014
食品.高效液相色谱法测定维生素A.第1部分:13视黄醇和全反视黄醇含量测定
Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 1: Measurement of all-E-retinol and 13-Z-retinol
EN 12823-1-2014 中，可能用到以下仪器设备
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- 标准号
- EN 12823-1-2014
- 发布日期
- 2014年05月
- 实施日期
- 废止日期
- 无
- 中国标准分类号
- X00
- 国际标准分类号
- 67.050
- 发布单位
- IX-CEN
- 引用标准
- EN ISO 3696-1995 EN ISO/IEC 17025-2005
- 被代替标准
- EN 12823-1-2000 FprEN 12823-1-2014
- 适用范围
- This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This method has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and -carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol. The extract obtained after saponification in this method can be used for the determination of -carotene, as described in EN 12823-2:2000, Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of -carotene. In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerisation and oxidation of -carotene.