肝癌体内外模型总结&工具病毒使用指南
肝癌是指发生于肝脏的恶性肿瘤，中国新增癌症病例高居世界第一位，其中肝癌的新增病例和死亡人数均居世界首位。目前，我国肝癌的发病率约为25.7/10万，成为死亡率仅次于胃癌、肺癌的第三大恶性肿瘤。探寻肝癌的生物学特性、发病及转移机制已成为当今研究的热点。
针对肝癌的研究少不了细胞和动物模型的加持。怎么选择这些工具能够帮助我们更好的进行研究，获得阳性数据，发表论文，申请基金，登上人生巅峰，你有了解吗？
一、我需要哪种细胞模型？是否易于操作？
因为细胞容易操作，时间周期短，容易出结果，所以肯定是所有研究的第一步，下面总结一下一些常用的肝癌细胞的特性：
*更多更全的预实验信息，可咨询当地业务员
二、细胞实验效果不错，那动物实验怎么做？
做完体外的细胞实验，要想发高分文章，必须要有体内部分的数据来证明，肝癌的动物模型不少，构建难度也各异，以下为各种方法的小结：
三、体内外模型选好了，那么我的实验需要使用哪种慢病毒载体对基因进行操作呢？
启动子、荧光标记、抗性标签是我们选择载体需要考虑的三大要素。实验不同，这些元件也需要相应变化。对于肝癌研究来说，hAAT1、ALB是较为常用的肝组织特异性启动子。按例祭出神图一张，定制你的专属载体，就是这么简单！
参 考 文 献
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