BS ISO 16649-1-2001 食品和动物饲料的微生物学.β-葡萄糖醛酸酶阳性大肠杆菌计数的水平方法.44 ℃下用隔膜和5-溴-4-氯-3-吲哚-β-D葡糖苷酸的菌落计数技术
Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of -glucuronidase-positive Escherichia coli - Colony-count technique at 44 C using membranes and 5-bromo-4- chloro-3-indolyl--D-glucuronide
This part of ISO 16649 specifies a horizontal method for the enumeration of β-glucuronidase-positive Escherichia coli in products intended for human consumption or for the feeding of animals. It uses a colony-count technique after resuscitation using membranes and incubation at 44 ℃ on a solid medium containing a chromogenic ingredient for detection of the enzyme β-glucuronidase.
WARNING —Strains of Escherichia coli which do not grow at 44 ℃ and, in particular, those that are β-glucuronidase negative, such as Escherichia coli 0157, will not be detected.