DNA标记
DNA标记（主要内容如下）
DNA Labeling by Nick Translation
Random Primed Labeling
End-Labeling
Purification of Labeled DNA
Non-isotopic Labeling
Others
DNA Labeling by Nick Translation
§ DNA Labeling by Nick Translation (Ambros Lab)
§ Nick Translation (Worthington)
§ Nick Translation (Promega)
§ Nick Translation (Life Technologies)
Random Primed Labeling
· Random Primer Labeling (Karl Clark)
Prepare radioactively labeled DNA strands for the detection of target DNA or RNA sequences in usr/localious applications including Southern and northern blot hybridization. Detailed protocol for labeling, purification and determination of incorporation
· Random Primed Labelling with P32 (Molecular Genetics Network Lab)
· Random Primer Labeling (Worthington)
· Random Primer Labeling (Crawford Lab)
· Random primer labeling (Amersham Pharmacia)
· Random Primer DNA Labeling (Life Technologies)
· Random Primer DNA labeling (Fermentas)
Labeling of DNA by random oligonucleotide-primed synthesis
· Random Hexamer Labeling of cDNA Probes (PMCI Research)
End-Labeling
· 5'-End Oligo Fluorescence Labeling (Amersham Pharmacia)
Labeling of oligonucleotides with a single fluorescein residue at the 5' end
· 5' end labeling of oligonucleotides with gamma-32P-ATP (Petra Klaff)
Labeling and purification method in great detail
· 3'-end Labeling (Promega)
· DNA Labeling using Klenow Fragment I (Lazo Lab)
· End-labeling of DNA fragments
Single end or double ends, can also be used for RNA
· Labeling of Oligonucleotides (PMCI Research)
Label oligo with polynucleotide kinase.
· Alkaline phosphatase labeling of oligos (Promega)
Provides useful guide for preparing phosphatase conjugated oligos
· Kinase End-Labeling of DNA (Roe, OU)
· Endlabeling of DNA fragments with Klenow polymerase (Molecular Genetics Network Lab)
· 5'-end Labeling (Amersham Pharmacia)
· Fill in Labeling of DNA (Mike A. Dyer)
· 5'-End Labeling (Fermentas)
T4 polynucleotide kinase labeling (ds and ss DNA or RNA; protruding, recessed and blunt 5'-ends) for (1)end labeling of DNA or RNA probes in DNA hybridization and DNA footprinting experiments, etc; (2)obtaining labeled molecular weight markers;(3)obtaining labeled molecular weight markers; (4)labelling of primers for sequencing; (5)addition of 5'-phosphates to oligonucleotides to allow subsequent ligation.
· Oligonucleotide labeling with 32P (Hahn Lab)
T4 polynucleotide kinase labeling
Purification of Labeled DNA
§ 5' end labeling of oligonucleotides and purification (Petra Klaff)
Labeling and purification method (ethanol and CTAB in great detail
§ Oligo Purification on Acrylamide Gels (Botwell Lab)
Purification of labeled oligos
· Sephadex G-50 Spin Column (gopher://iubio.bio.indiana.edu/)
For purifying labeled probe
· Spin Column chromatography (Schimenti Lab)
Used to removed unincorporated nucleotides from labeling reactions.
· Oligonucleotide Purification by PAGE and DEAE (Hahn Lab)
· PAGE Purification of Oligonucleotides (Life Technologies)
Protocol for PAGE Purification of Oligonucleotides Protocol for PAGE Purification of Oligonucleotides Oligonucleotides contaminated by significant amounts of aborted synthesis products (e.g., n-1,...
· PCR DNA Biotinylation (KPL)
A rapid method for biotinylating DNA probes through incorporation of biotin-N4-dCTP via a thermostable DNA polymerase in the polymerase chain reaction
· Determination of efficiency of digoxigenin labelling (Molecular Genetics Network Lab)
· Non-isotopic Labeling (Gottschling Lab)
Protocols for preparing DNA, RNA probe and molecular marker
· PCR Products Labeling (Amersham)
Guidelines for preparation of radioactively-labeled PCR products for use as probes
