This European Standard specifies methods for the determination of residues of pesticides and polychlorinated biphenyls (PCBs) in fatty food. Each method described in this European Standard is suitable for identifying and quantifying a definite range of those non polar organochlorine and/or organophosphorus pesticides which occur as residues in fats and oils as well as in the fat portion of fat containing foodstuffs, both of either animal or vegetable origin. The PCB indicator congeners usually selected for the enforcement of maximum residue limits (MRLs) are determined along with the organochlorine pesticides.
This European Standard contains the following clean-up methods that have been subjected to interlaboratory studies and are adopted throughout Europe.
- Method A. Liquid—liquid partitioning with acetonitrile and clean-up on a Florisil column (AOAC) .
- Method B. Liquid—liquid partitioning with dimethylformamide and clean-up on a Florisil column (Specht) .
- Method C. Column chromatography on activated Florisil(AOAC) .
- Method D. Column chromatography on partially deactivated Florisil(Stijve) .
- Method E. Column chromatography on partially deactivated aluminium oxide (Greve & Grevenstuk) .
- Method F. Gel permeation chromatography (GPC) (AOAC) .
- Method G. Gel permeation chromatography (GPC) and column chromatography on partially deactivated silica gel (Specht) .
- Method H. High performance gel permeation chromatography (HPGPC) (MAFF) .
The applicability of the eight methods A to H for residue analysis of organochlorine pesticides, PCB indicator congeners, and organophosphorus pesticides, respectively, is given in table A.1. Where no + sign is shown, there are no data available in literature, but this does not necessarily exclude the applicability.
note:1) Florisil is an example of a suitable product available commercially. This information is given for the convenience of users of this European Standard and does not constitute an endorsement by CEN of the product named.