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如何有效地降低PepMuteTM,GenMuteTM and PepMuteTM Plus siRNA转...

2020.5.27

如何有效地降低PepMuteTM,GenMuteTM and PepMuteTM Plus siRNA转染试剂的毒性


PepMuteTM,GenMuteTM 及 PepMuteTM Plus转染试剂,仅被发现在DMEM基础细胞培养基中偶有毒性,使用其他的培养基(含10%FBS及抗生素)培养细胞,将基本减少转染中的毒性。譬如,如果您发现有大量细胞死亡,您可能使用含10%FBS及抗生素的DMEM基础培养基。要消除毒性,您可以选择DMEM/F12或者RPMI 1640来替代DMEM作为基础培养基。至于为什么,到目前为止,我们也是不得而知,但是,它却有效地减少了毒性。试试吧!

How to eliminate toxicity of PepMute™, GenMute™ and PepMute™ Plus siRNA Reagents

PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium.  Using other cell culture mediums (supplemented with 10% FBS and antibiotics) to grow your cells will basically eliminate the transfection toxicity. For instance, if you find toxicity and high cell death, you are probably using DMEM base medium supplemented with 10% FBS and antibiotics to grow your cells.  To remove the transfection toxicity, you can change DMEM to DMEM/F12 or RPMI 1640 as base medium.   So far we have not figured out why, but it does minimize the toxicity.  Try it out!


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