T载体的制作和应用
Also see DNA Cloning
§ Making TA Vector (Crawford Lab)
T-vectors are linear-blunt-ended plasmids with a few dT's added on by Taq polymerase.
§ Making TA Vector (Mounir Izallalen)
§ Direct PCR cloning (Hoshi Lab)
Cloning PCR products directly to an expression vector
§ TA Cloning (WUGSC)
TA cloning ligation reaction and subsequent transformation by electroporation.
§ TA Subcloning of PCR Products (Gerard Lazo)
§ Ligation (Life Technologies)
Rapid ligation protocol for plasmid cloning
§ Ligation (Crawford)
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