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ProZyme公司推出Glycan-Q系统用于综合的N-糖分析

2016.11.14

　　分析测试百科网讯，位于加利福尼亚州海沃德的ProZyme公司，糖生物学领先的试剂和工具制造商，2016年11月14日宣布推出Glycan-Q糖分析系统, 一个集成的高通量平台, 可进行用户友好的糖基分析。Glycan-Q系统利用ProZyme在快速糖样品制备和分析级糖基标准品方面的专长, 并结合小型毛细管凝胶电泳 (CGE) 仪器，后者装载有为简化糖数据分析而设计的定制化软件。

　　"我们很高兴推出ProZyme 的第一款仪器产品, " ProZyme 的总裁兼首席执行官Sergey Vlasenko博士说。"我们在过去几年中加快了糖样品制备的工作, 但数据分析仍然是一个瓶颈。通过我们的新系统, 生物制药公司的细胞线开发和分析组可以更快地确定样品的糖谱, 同时降低总体成本。我相信该系统可以帮助研究人员运行 glycomic（糖组学）分析的大型数据集, 包括临床样本。

　　蛋白质糖基化是一种翻译后修饰, 可影响治疗蛋白的有效性和安全性, 如对单克隆抗体, N-糖基化是一个关键的质量属性。蛋白质糖基化也可以作为特定疾病状态的指示。传统的N-糖分析方法包括缓慢的、耗费大量人力的步骤来进行样品制备、糖分离和数据分析。ProZyme 推出了Glycan-Q系统，实现了糖分析的高通量和简单化，可用于生物治疗药物的开发, 并帮助发现糖生物标志物。

　　在Glycan-Q工作流的前端, ProZyme Gly-X化学酶和PNGase（聚糖酶）的作用下，可5分钟内从糖蛋白中释放N-糖，释放出的N-糖立即用荧光染料标记, 然后用CGE分离。整个样品准备过程可在45分钟内完成, 并且是自动化的。荧光标记的N-糖然后在Glycan-Q CGE 仪器上分离, 每个样本运行时间为2分钟, 允许在一个8小时的工作日内准备和分析96个样本。该仪器具有易于更换的Glycan-Q卡盒, 含有毛细管和凝胶基质。

　　Glycan-Q仪器控制和N-糖数据分析由定制构建的Glycan-Q管理器软件完成。电泳标准品是与每个样本共同注入的, 这与阶梯标准品一起实现了对每个样本峰值的自动数据对准和葡萄糖单位 (GU) 值的计算。然后将样本的GU值与内置和可自定义的糖参考库进行比较, 以进行峰值分配。通过 ProZyme 的糖标准品和外切糖苷（exoglycosidase）来增强分析效果。

　　Gly-Q系统用于N-糖样品制备、分离和分析，由ProZyme 销售。要了解更多关于Gly-Q系统的信息, 请访问 prozyme.com:

　　Gly-Q系统糖分析系统

　　Gly-Q管理者软件

　　Gly-X N-糖快速释放，InstantQ 套件标记

关于ProZyme

　　ProZyme, Inc. 创立于1990年，其愿景是建立一家高品质、有附加值、客户驱动的生化试剂公司。25年来，我们自豪地为世界各地的生物技术、生物制药和学术机构提供最高水准的产品、服务和支持。ProZyme的旗舰产品线是我们的糖生物学产品阵容，以及链亲和素、藻胆蛋白和偶联物，我们还与我们的客户合作开展定制分析服务。ProZyme恪守承诺，即开发、交付、支持糖生物学这一激动人心而迅速扩张领域中的各类产品。

　　英文原文：

　　ProZyme, Inc. Launch Gly-Q System For Integrated N-Glycan Analysis

　　Published: Nov 15, 2016

　　HAYWARD, Calif., Nov. 14, 2016 /PRNewswire-iReach/ --ProZyme, Inc., a leading manufacturer of reagents and tools for glycobiology, today announced the launch of the Gly-Q Glycan Analysis System, an integrated platform for high-throughput, user-friendly glycoanalysis. The Gly-Q System will leverage ProZyme's expertise in rapid glycan sample preparation and analytical glycan reference standards, combined with a small capillary gel electrophoresis (CGE) instrument powered by custom software for simplified glycan data analysis.

　　Photo -http://photos.prnewswire.com/prnh/20161114/438816

　　"We are delighted to introduce ProZyme's first instrument offering," said Sergey Vlasenko, Ph.D., President and CEO of ProZyme. "We have led the way in speeding up glycan sample preparation in the past few years, but data analysis has still been a bottleneck. With our new system, cell line development and analytical groups at biopharma companies can determine the glycan profiles of their samples faster, while reducing overall costs. I believe this system could benefit researchers running glycomic analysis of large datasets including clinical samples."

　　Protein glycosylation is a post-translational modification that can influence the efficacy and safety of therapeutic proteins such as monoclonal antibodies, and N-glycosylation can be a critical quality attribute. Protein glycosylation can also serve as a signature of a specific disease state. Traditional methods for N-glycan analysis involve slow and labor-intensive steps for sample preparation, glycan separation and data analysis. ProZyme introduces the Gly-Q system to enable high-throughput and accessible glycan analysis for use in biotherapeutic development, and to aid in glycan biomarker discovery.

　　At the front end of the Gly-Q workflow, ProZyme Gly-X chemistry enzymatically releases N-glycans from glycoproteins with PNGase F in 5 minutes, and released N-glycans are instantly labeled with a fluorescent dye that enables separation by CGE. The entire sample preparation protocol can be completed in as little as 45 minutes, and is automatable. Fluorescently-labeled N-glycans are then separated on the Gly-Q CGE instrument with a run time of 2 minutes per sample, allowing 96 samples to be prepared and analyzed within a single 8-hour work day. The instrument features an easily-replaceable Gly-Q cartridge containing a capillary and gel matrix.

　　Gly-Q instrument control and N-glycan data analysis is facilitated by custom-built Gly-Q Manager software. Migration standards are co-injected with each sample, which along with a ladder standard enables automatic data alignment and the calculation of glucose unit (GU) values for each sample peak. GU values for samples are then compared against built-in and customizable N-glycan reference libraries for peak assignment. Analysis is enhanced with ProZyme's glycan reference standards and exoglycosidase enzymes.

　　The combined Gly-Q System for N-glycan sample preparation, separation and analysis is exclusively marketed by ProZyme worldwide. To learn more about the Gly-Q System, please visit prozyme.com:

　　Gly-Q Glycan Analysis System

　　Gly-Q Manager Software

　　Gly-X N-Glycan Rapid Release & Labeling with InstantQ Kit

　　About ProZyme

　　ProZyme, Inc. is a privately held company based in Hayward CA founded in 1990 with a vision of establishing a high-quality, value-added and customer-driven biochemical reagent company. We are proud, 26 years on, to provide the highest level of products, services and support to our customers at biotechnology, biopharmaceutical and academic institutions across the world. ProZyme's flagship product line is an extensive glycobiology portfolio, alongside streptavidin, phycobiliprotein and conjugate offerings. We perform custom analytical services, and welcome the opportunity to collaborate with our customers. ProZyme maintains a commitment to develop, release and support products in the exciting and rapidly-expanding area of glycobiology.

　　Media Contact:Aled Jones, ProZyme, Inc., (510) 638-6900, info@prozyme.com

　　News distributed by PR Newswire iReach: https://ireach.prnewswire.com

　　SOURCE ProZyme, Inc.

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