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激光扫描影像系统在3D肿瘤球体或干细胞克隆球体快速...2

2020.6.01

结果：

肿瘤微球体生长在超低粘附板中

A 96孔板
	Figure 2A: 生长在96孔超低粘附板中的HepG2肿瘤微球体的全孔TIFF图像，图像底部显示了加入每孔的阿霉素浓度。2B-C：药物量效曲线（mean±SEM，n=6）
B	C

A 384孔板
	Figure 3A:生长在384孔超低粘附板中的HepG2肿瘤微球体的全孔TIFF图像，图像底部显示了加入每孔的阿霉素浓度。3B-C：药物量效曲线（mean±SEM，n=8）
B	C

肿瘤微球体生长在软琼脂中

A	B	C

D
	Figure 4A: 生长在96孔板软琼脂中的HepG2肿瘤微球体的全孔TIFF图像，图像上显示了加入每孔的阿霉素浓度。4B-D：药物量效曲线（mean±SEM，n=3）

A	B	C

D
	Figure 5A: 生长在384孔板软琼脂中的HepG2肿瘤微球体的全孔TIFF图像，图像上显示了加入每孔的阿霉素浓度。5B-D：药物量效曲线（mean±SEM，n=4）

总结：

生长在 3D培养系统中的细胞群体、不同细胞类型、微组织结构相互协调、相互作用，模拟了生命物质在体内微环境中的真实生存环境，为研究者对疾病的剖析和攻克提供了高质量的数据信息。我们在96和384微孔板中建立了两种简单且稳定的进行肿瘤微球体3D培养的方法，通过染色和图像采集，获得数目、面积、体积和荧光强度等信息。药物量效曲线的一致性结果体现了两种方法的可靠性和可重复性，这些数据表明acumen hci激光扫描系统是对基于两种培养系统获得的肿瘤微球体进行高通量（5分钟/板）分析的理想平台，该平台在过去的研究中已经被广泛地应用于肿瘤学[7-9]和干细胞研究领域[10-12]。

References：

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